Synergistic activity of Herbal Extracts on Dog Blood Progenitor cells

Peripheral blood is an accessible adult stem cell source for both basic and applied research including clinical applications. Peripheral blood mononuclear cells (PBMCs) were reported to possess multipotent progenitor cell populations and has the potential to differentiate into blood cells, endothelial cells, hepatocytes, cardiomyogenic cells, smooth muscle cells, osteoblasts and so on. They can be used to predict in vivo toxicity only if independent exposure and pharmacokinetic disposition and metabolism studies are available. In the present study adaptogenic herbs with beneficial properties such as Withania somnifera, Ocimum sanctum, Phyllanthus amarus were tested for inducing proliferative activity on blood derived stem cell population. It was observed that the blood progenitor cells treated with tested herbs were not toxic and there were no significant adverse effects on the livability of cultured cells during the observation period. The extracts of Withania somnifera, Ocimum sanctum, Phyllanthus amarus showed synergistic activity on stimulation of cell proliferation.